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KMID : 0368420050480010085
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Journal of Plant Biology
2005 Volume.48 No. 1 p.85 ~ p.95
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Low expression profiles of heat stress-related genes in Capsicum annuum
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Ashrafuzzaman M.
Oh S.-June
Hong Choo-Bong
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Abstract
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A cDNA library was constructed for hot pepper plants that had been heat-shock-treated. We used a modified differential screening method, double negative screening, to isolate 500 cDNA clones that represented genes with low expression levels under conditions of high-temperature stress. Of those 500 clones, 200 were randomly selected for single-read sequencing from the 5′ ends. After annotation with Blastx, the sequence was applied to InterProScan to scan for functional motifs of proteins. Among the cDNA clones analyzed, about 41% of the ESTs could not be functionally classified. However, of those that could be, the largest portion of the ESTs (15%) were assigned to the category of cell rescue and defense; genes involved in cell cycle/DNA processing constituted the smallest group, comprising 1% of the ESTs. Genes related to energy and protein fates constituted the second (10%) and third (9%) largest groups, respectively. Finally, 3% of the ESTs were assigned to transcription, and 2% to signal transduction. The high portion of unclassified ESTs probably resulted from the screening method, which was designed for low-expression messages. Likewise, the high number of ESTs for cell rescue and defense suggests that many genes with low levels of expression are associated with the stress response.
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KEYWORD
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cDNA library, double negative screening, high-temperature stress, hot pepper (Capsicum annuum)
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